Anti-obesity effects of dichloromethane leaf extract of Gnidia glauca in high fat diet-induced obese rats

Abstract

Obesity is a chronic metabolic disorder characterized by increased adipose tissue mass due to positive energy balance. Prescription of anti-obesity drugs can be useful adjuncts to diet and exercise for obese patients who have failed to achieve weight loss. However, these drugs are ineffective and are associated with adverse effects. In recent times, medicinal plants have drawn a sharp focus owing to their biocompatibility and effectiveness. Attempts to determine the therapeutic effects and identification of bio-active principles from herbal prescriptions have become the prime focus in the validation of their folkloric usage and in drug discovery programs. Therefore, the present study aimed to determine the anti-obesity effects of Dichloromethane leaf extract of Gnidia glauca in high-fat-diet-induced obese rats. Obesity was induced experimentally in white albino Wistar rats by feeding them with prepared high-fat diet and water ad libitum for a period of 12 weeks. The in-vivo anti-obesity effects were determined by oral administration of Gnidia glauca at dosage levels of 200, 250 and 300 mg/kg body weight from the 6th to 12th week of study. Phytochemical analysis of Gnidia glauca was conducted using gas chromatography linked to mass spectrophotometer. The results indicated that Gnidia glauca exhibited potent anti-obesity effects. It significantly reduced the body weight, organ weights, organo-somatic indices, anthropometric indices, the total fat content, adiposity index, atherogenic index as well as various lipid profiles. It also decreased the total feed intake. However, it significantly increased levels of high-density lipoproteins and rectal body temperature of rats. Quantitative phytochemical analysis also revealed the presence of various phytocompounds that have shown to be associated with anti-obesity effects. The anti-obesity effects of Gnidia glauca may be attributed to the phytochemicals present. The present study, therefore, scientifically validates the traditional use of Gnidia glauca as a potential candidate for the synthesis of new effective anti-obesity supplement.