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    PfEMP1 DBLα Sequence Tags in Genomic DNA of P. falc iparum Field Isolates from Two Malaria Endemic Sites in Ke nya

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    Date
    2015
    Author
    Makokha, Francis W.
    Omar, Sabah A.
    Kimani, Francis T.
    Magoma, Gabriel
    Udu, Rahma
    Too, Edwin
    Shaviya, Nathan
    Hungu, Charity
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    Abstract
    Malaria caused by Plasmodium falciparum remains a major cause of childhood morbidity and mortality in sub-Saharan Africa. PfEMP1 protein, coded for by a family of about sixty variant var genes, is a parasite protein found on infected erythrocyte membrane. PfEMP1 protein mediates cytoadherence of infected erythrocytes on endothelial cells which may lead to severe symptoms of malaria. Although PCR amplification of the whole gene is difficult due to high variability, primers targeting the DBLα domain have been designed and used to study pfemp1 genes. This objective of this study was to establish the distribution of DBLα sequence tags in isolates of Plasmodium falciparum from two malaria endemic sites in Kenya.
    URI
    http://hdl.handle.net/123456789/7922
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