In Vitro Plant Regeneration of Sweetpotato Through Direct Shoot Organogenesis
Date
2018-04Author
Makenzi, Nzaro G
Mbinda, Wilton M
Okoth, Richard Oduor
Ngugi, Mathews Piero
Metadata
Show full item recordAbstract
Sweetpotato (Ipomoea batata) is an important, nutritionally rich vegetable crop, but severely affected by
environmental stresses, pests and diseases which cause massive yield and quality losses. Genetic manipulation
is becoming an important method for sweetpotato improvement. In the present study, a reproducible and
highly efficient protocol for in vitro plant regeneration of six Kenyan farmer preferred sweetpotato, Enaironi,
KEMB 36, KSP36, Mugande, Kalamb Nyerere, SPK 013 and SPK004 through direct shoot organogenesis from
stem internodes explants was developed. The results revealed that Kalamb nyerere had the highest number of
adventitious bud; for light (5.33 and 4.33) and dark (8.00 and 5.00) induction condition for all TDZ hormone
level (0.25 mg/l and 0.15 mg/l). When explants incubated in 0.10 mg/l NAA the regeneration frequencies were the
highest at 83.33% (Jewel) and 96.67% (Kalamb nyerere) for adventitious buds recovered from light and darkness
respectively. This was the optimal auxin concentration which gave the maximum regeneration frequency with
adventitious buds recovered from the dark. The best Kenyan sweetpotato genotypes for direct shoot
organogenesis were Kalamb nyerere, Kemb 36 and SPK 004. The protocol presented in this work is suitable for
improvement of sweetpotato genotypes through tissue culture methods and or genetic transformation