dc.contributor.author | Walekhwa, Michael | |
dc.contributor.author | Muturi, Margaret | |
dc.contributor.author | Gunturu, Revathi | |
dc.contributor.author | Kenya, Eucharia | |
dc.contributor.author | Kabera, Beatrice | |
dc.date.accessioned | 2024-03-19T06:36:22Z | |
dc.date.available | 2024-03-19T06:36:22Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Walekhwa, M., Muturi, M., Gunturu, R., Kenya, E., & Kabera, B. (2018). Streptococcus pneumoniae serotype epidemiology among PCV-10 vaccinated and unvaccinated children at Gertrude’s Children’s Hospital, Nairobi County: a cross-sectional study. F1000Research, 7. | en_US |
dc.identifier.other | doi: 10.12688/f1000research.14387.2 | |
dc.identifier.uri | http://ir.tum.ac.ke/handle/123456789/17543 | |
dc.description | doi: 10.12688/f1000research.14387.2 | en_US |
dc.description.abstract | Background: Serotype replacement and emergence of multidrug resistant S.
pneumoniae has exacerbated the need for continuous regional serotype
surveillance especially in the developing world. We investigated S. pneumoniae
serotypes circulating among vaccinated and unvaccinated children ≤5 years in
Nairobi County post PCV10 era.
Methods: A total of 206 vaccinated and unvaccinated children attending
Gertrude’s Children’s Hospital (GCH) were recruited for this study.
Nasopharyngeal swabs collected using Copan Flocked Swabs were the main
study specimen. Culturing and isolation of S. pneumoniae was done on BA with
gentamicin and BA plates respectively at the GCH main laboratory. Serotyping
was done using the Quellung reaction at the KEMRI-Wellcome Trust, Kilifi.
Results: Out of the 206 subjects sampled, 20.39% (42) were found to be
carriers of S. pneumoniae. About 52% (n=22) of the S. pneumoniae carriers
had received the recommended dose of PCV-10, while 48% (n=20) of the
carriers had not. Almost all (n=41; 19.90% of subjects) isolates contained
non-vaccine type S. pneumoniae serotypes, while n=1 of the serotypes (in
0.49% of subjects) were untypeable. Serotypes 28F, 6A, 11A, 3 and 7C were
prevalent in both vaccinated and unvaccinated children, whereas serotypes
23A, 17F, 35F, 48, 13 and 35B, and 23B, 20, 19B, 21, untypeable, 15B and 39
were found among unvaccinated and vaccinated groups, respectively.
Conclusions: All S. pneumoniae serotypes isolated from the subjects sampled
were non PCV-10 vaccine type. These results therefore highlight the
importance of monitoring and evaluation to provide epidemiological information
to determine the effectiveness of PCV10 in Kenya’s Public health services. | en_US |
dc.description.sponsorship | TECHNICAL UNIVERSITY OF MOMBASA | en_US |
dc.language.iso | en | en_US |
dc.publisher | Faculty of 1000 Ltd | en_US |
dc.subject | Streptococcus pneumoniae | en_US |
dc.subject | Nairob | en_US |
dc.subject | serotypes | en_US |
dc.subject | Quellung reaction | en_US |
dc.subject | Optochin test | en_US |
dc.subject | Bile solubility | en_US |
dc.title | Streptococcus pneumoniae serotype epidemiology among PCV-10 vaccinated and unvaccinated children at Gertrude’s Children’s Hospital, Nairobi County: a cross-sectional study | en_US |
dc.type | Article | en_US |