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    Canine leishmaniasis caused by Leishmania leishmania infantum in two Labrador retrievers

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    Awadh 3.pdf (172.3Kb)
    Date
    1992
    Author
    Awadh A. Binhazim
    Willie L. Chapman
    Jr., Kenneth
    S. Latimer
    Martha Styles
    Karen Comer
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    Abstract
    Canine leishmaniasis, a generally fatal parasitic disease, was diagnosed in 2 dogs with a medical history of foreign travel, lymphadenopathy, emaciation, anorexia, intermittent fever, and cutaneous lesions. Clinically, hyperproteinemia, proteinuria, azotemia, and glomerulopathy were evident. Isolation of Leishmania species was done using Schneider’s Drosophila medium. Syrian hamsters were used for infectivity studies. Clear taxonomic identification was done biochemically by isoenzyme analysis and comparison of zymogram banding patterns with 6 World Health Organization reference strains. Based on the geographic origin of affected dogs, clinicopathologic presentation, visceralization with hepatosplenomegaly in hamsters, and isoenzyme analysis, a diagnosis of Leishmania leishmania infantum was made. This study, representing the first taxonomic identification of an isolate from canine leishmaniasis, demonstrates the Zoonotic and epidemiologic implications of this disease.
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    http://hdl.handle.net/123456789/5528
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