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dc.contributor.authorFengling Luo
dc.contributor.authorYong Feng
dc.contributor.authorMin Liu
dc.contributor.authorPingfei Li
dc.contributor.authorQin Pan
dc.contributor.authorVictor Tunje Jeza
dc.contributor.authorBing Xia
dc.date.accessioned2015-11-02T07:35:32Z
dc.date.available2015-11-02T07:35:32Z
dc.date.issued2007
dc.identifier.urihttp://hdl.handle.net/123456789/5546
dc.descriptionThe original publication is available at http://journals.asm.org/en_US
dc.description.abstractAttenuated Salmonella enterica serovar Typhi strains have been considered to be attractive as potential live oral delivery vector vaccines because of their ability to elicit the full array of immune responses in humans. In this study, we constructed an attenuated S. enterica serovar Typhi strain stably expressing conserved nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) by integrating the N gene into the pilV gene, which was under the control of the type IVB pilus operon promoter in S. enterica serovar Typhi. BALB/c mice were immunized with this recombinant strain through different routes: intranasally, orogastrically, intraperitoneally, and intravenously. Results showed that the intranasal route caused the highest production of specific immunoglobulin G (IgG), IgG2a, and secretory IgA, where IgG2a was imprinted as a Th1 cell bias. Moreover, this recombinant live vaccine induced significantly high levels of specific cytotoxic T-lymphocyte activities and increased gamma interferon-producing T cells compared with the parental strain. Our work provides insights into how the type IVB pilus operon promoter controlling SARS-CoV N gene expression in Salmonella might be attractive for a live-vector vaccine against SRAS-CoV infection, for it could induce mucosal, humoral, and cellular immune responses. Our work also indicates that the type IVB pilus operon promoter controlling foreign gene expression in Salmonella can elicit full immune responses by intranasal vaccination.en_US
dc.description.sponsorshipTechnical University of Mombasaen_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectpilus operon promoteren_US
dc.subjectcoronavirus nucleocapsid geneen_US
dc.subjectSalmonella enterica Serovar Typhi elicitsen_US
dc.titleType IVB Pilus Operon Promoter Controlling Expression of the Severe Acute Respiratory Syndrome-Associated Coronavirus Nucleocapsid Gene in Salmonella enterica Serovar Typhi Elicits Full Immune Response by Intranasal Vaccinationen_US
dc.typeArticleen_US


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